Johnathan Joestar - Bisexual

Speedwagon - Gay (semi-canon)

William Zeppeli - Straight

Dio Brando - Bisexual (canon)

Joseph Joestar - Bisexual

Caesar Zeppeli - Gay

Kars - Asexual

Wammu - Gay

Esidisi - Pansexual

Jotaro Kujo - Straight

Noriaki Kakyoin - Pansexual

Mohammad Avdul - Gay

Jean Pierre Polnareff - Bisexual

Vanilla Ice - Gay

Josuke Higashikata - Gay

Okuyasu Nijimura - Bisexual

Akira Otoishi - Asexual

Rohan Kishibe - Asexual

Yoshikage Kira - Straight

Giorno Giovanna - Gay

Bruno Bucciarati - Gay

Leone Abbacchio - Gay

Guido Mista - Bisexual

Narancia Ghirga - Gay (femboy too, why not)

Panacota Fugo - Gay

Trish Una - Asexual

Doppio - Bisexual

Diavolo - Bisexual

Risotto Nero - Asexual

Squalo and Tiziano - GAYS, THEYRE FKING GAYS

Jolyne Cujoh - Pansexual

Ermes Costello - Lesbian

Narciso Anasui - Straight (transmasc too)

Weather Report - Straight

Foo Fighters - Asexual non-binary, they're a bunch of planktons

Enrico Pucci - Gay

Johnny Joestar - Bisexual

Gyro Zeppeli - Gay

Diego Brando - Bisexual

Hot Pants - Pansexual

Lucy Steel - Bisexual

Funny Valentine - Pansexual

Wekapipo - Gay

Sandman - Asexual

Jesus Christ - Jesus Christ

Josuke 8 - Bisexual

Yasuho Hirose - Bisexual

Joubin Higashikata - Straight

Tooru - Pansexual