As of this post 20 characters have been programmed in total, the new ones are:

Brundle: When he appears in the office, click his hand to give him a high five, not doing so makes him shoot a laser at you, dealing damage.

U.T.: If the camera is open, there is a chance two UFO’s will spawn, click on both before they reach the end of their patch. This summons U.T. into the office, killing you.

Mime: He stands in the office and occasionally points up, left, right, or down. Each pose signifies an action you have to do, like opening the camera or closing a door. Not doing what he’s doing will make him angry, and your reward is a mime murdering you.

Stalker: His long eye stalks lurk on the other side of the desk, slowly inching out further. Put the mouse over them to make them hide back into the desk. Letting his eyes peek just a little too much costs you your life.

Blob: He can appear in either hall, close the respective door for the hall he’s in.

Wash-R: He attacks from the right hall, close the door when he’s there.

Frundy Fezburger (The totally original character!): He acts like Wash-R, but from the right. He is slower, so he won’t appear as much, but he’ll stick at the door longer.

Batte: She appears in the generator room and drains power, shock her to stop her.

Shock: He appears in the generator room as well. If you check the room when he’s in it, he’ll stun you, disabling doors and the camera for a while. To avoid this, hover over the camera button for the generator room (CAM3) and wait if you hear a grating electrical noise. This signifies that he’s there, so do not click the button until the noise is gone.

Gelno: He is the first of the vent crawlers, when he appears close to the office in the monitor, close the vent. You can slow him down with audio lures and vent snares.