Manic: He is the main character, he is unlocked from the beginning of the game.

Luils: He is unlocked after completing the game

Mangle & Billy: They are unlocked after completing the game

Sonic: Unlocked after completing the EXE's challenge

Mini MANIC: unlocked after completing the game 2 times with the 101% of the tower

Foxy: He is unlocked after completing the game without taking damage in his boss battle.

Springtrap/Afton: He is unlocked after completing the game without taking damage in his boss battle.

Metal Manic: He is unlocked after completing the game without taking damage in his boss battle.

X: He is unlocked after completing the game without taking damage in his boss battle.

Chef Metal: You have to get all the clothes and then win the Metal Manic battle without taking damage

Chef Manic: You have to complete all the achievements and then get a P-Rank in the Champion's Road

Super MANIC: He is only playable in THE CRUMBLING TOWER OF CHILLI